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METHOD DETAILS Sample preparation FOXO4 FHD (95-195) was subcloned into the pET His6 Tobacco Etch Virus (TEV) LIC cloning vector (2B-T, obtained from Scott Gradia, Addgene Plasmid #29666) and transformed into BL21 (DE3) cells
One dropper daily Use one full dropper (about 1 mL) per day
A provider-supervised taper or switch to oral B12 supplementation is a reasonable alternative if MIC B12 proves unsuitable
The drug is billed through the pharmacy benefit instead
No bad effects, normal blood work, and the drug cleared from the blood within 24 hours
The primary outcomes were the percentage weight change and the proportion of participants losing at least 5% of their baseline weight, using treatment-policy estimand analysis (intention-to-treat principle)
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