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Central to this is the ability of the copper ion to transition between the oxidation states Cu(II) and Cu(I)

GHK-Cu peptide is used for four main goals

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This process, along with GCL inhibition and the formation of mixed disulfides between GSH and protein sulfhydryl, concurred to a steady-state decrement of free GSH concentration and, consequently, to an intracellular redox state shift toward more oxidizing conditions, which could be required for avoiding fast reduction of oxidized cysteines allowing the upstream ROS-induced autophagic stimulus to be propagated

Hadamard Encoding and Reconstructions of MEGA-edited Spectroscopy HERMES, which was used in this study), allows us to differentiate GSH from other overlapping signals, providing better specificity and accuracy of the measured metabolite levels, and thus increased reliability of our results