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In an insulin-resistant milieu, insulin may competitively inhibit IDE, which impedes the degradation of A proteins, increases their neurotoxicity, and contributes to the onset of AD (Scherer et al., 2021
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The receptor was first incubated with the isolated G protein and, when applicable, the ligand (10 M) for 30 min in a 25 mM HEPES, 100 mM NaCl, 5 mM MgCl 2 , pH 7.5 buffer
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